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Image Search Results
Journal: Oxidative medicine and cellular longevity
Article Title: Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy.
doi: 10.1155/2021/3899356
Figure Lengend Snippet: Figure 5: rhGH decreased oxidative stress in db/db mice. (a) ROS-stained (red fluorescence) and DAPI-stained (blue fluorescence) photomicrographs. Scale bar: 100 μm, n = 3 per group. ∗∗P < 0:01 vs. db/db+rhGH group and #P < 0:01 vs. db/db group. (b) Cardiac MDA and GSH activities were quantified using commercial assay kits. n = 8 per group. ∗P < 0:05 vs. db/db+rhGH group and #P < 0:05 vs. db/db group. (c) Cardiac NRF2, HO-1, SOD, NOX4, and CAT levels were measured by western blotting. (d) Relative protein expression was quantified. n = 3 per group. ∗P < 0:05 vs. db/db+rhGH group, &P < 0:05 vs. db/db group, and #P < 0:01 vs. db/db group.
Article Snippet: The primary antibodies were against ANP (rabbit anti-ANP antibody, 1 : 500; Invitrogen, USA), BNP (rabbit anti-BNP antibody, 1 : 500; Invitrogen), CD36 (rabbit anti-CD36 antibody, 1 : 1000; Proteintech), SCD-1 (rabbit anti-SCD-1 antibody, 1 : 1000; Abcam), PGC1-ɑ (rabbit anti-PGC1-ɑ antibody, 1 : 1000; Proteintech), CPT-1 (rabbit anti-CPT-1 antibody, 1 : 1000; Proteintech), p-AMPK (rabbit anti-p-AMPK antibody, 1 : 1000; Proteintech), NRF2 (rabbit anti-NRF2 antibody, 1 : 500; Proteintech), HO-1 (rabbit anti-HO-1 antibody, 1 : 1000; Abcam), PPAR-α (rabbit anti-PPAR-α antibody, 1 : 500; Proteintech), SOD (rabbit anti-SOD antibody, 1 : 2000; Proteintech),
Techniques: Staining, Western Blot, Expressing